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Amoebic Antibodies

Amoebic Antibodies

The amoebic antibody test is an essential diagnostic tool for detecting infections caused by the protozoan parasite *Entamoeba histolytica*, particularly in cases of invasive amebiasis. This condition occurs when the parasite spreads from the intestines to other organs, such as the liver, causing abscesses and other complications. The test is especially useful when direct detection methods, like fecal microscopy, are inconclusive. It employs an Enzyme-Linked Immunosorbent Assay (ELISA) to identify IgG antibodies in the serum, indicating past or current infections. The procedure involves collecting a serum sample, which is then analyzed using ELISA to detect specific antibodies. Results are categorized as negative, equivocal, or positive, aiding in the diagnosis of extraintestinal complications. While serology is valuable for diagnosing invasive disease, it should be interpreted alongside clinical findings and other diagnostic methods. Proper specimen handling and storage are crucial for accurate results, with a typical turnaround time of 1 to 5 days.

Price of test:£65

The lowest price from the last 30 days: £65

Awaiting result:

One day

Collect material:

Blood

Prepare:

Learn more

Purpose of the test

The lab test for amoebic antibodies is essential for diagnosing infections by *Entamoeba histolytica*, especially in invasive amebiasis cases. This test is crucial when direct detection methods are inconclusive. It uses ELISA to detect IgG antibodies, indicating past or current infections. Key steps include:

- Sample Collection: Serum is collected, centrifuged, and stored properly.
- ELISA Procedure: Involves antigen-coated microplates and colorimetric detection.

Results interpretation:

- Negative: No infection.
- Equivocal: Follow-up needed.
- Positive: Suggests infection.

This test aids in diagnosing extraintestinal complications, complementing other diagnostic methods.

Who Should Get Tested

The lab test for amoebic antibodies is essential for diagnosing infections by *Entamoeba histolytica*, especially in cases of invasive amebiasis. This condition occurs when the parasite spreads beyond the intestines, affecting organs like the liver and lungs. The test is crucial when direct detection methods are inconclusive. It uses ELISA to detect IgG antibodies, requiring a serum sample. Results are interpreted as:

- Negative: No infection.
- Equivocal: Inconclusive; retest may be needed.
- Positive: Suggests current or past infection.

Proper specimen handling and storage are vital for accurate results.

Blood Test Preparation Guidelines

Time of day

It is recommended to schedule your appointment for blood tests in the morning hours between 7:00-10:00.

Fasting

It is recommended to fast for approximately 12 hours before blood sampling. The last meal of the previous day should ideally be consumed around 6:00 p.m. On the day before the test, avoid heavy and fatty meals as well as alcohol.

Stay hydrated

Drinking water prior to testing can help with sample collection.

Other factors

Tests should not be performed after a sleepless night or intense physical activity. It is recommended to avoid exercise and stress immediately before blood collection and to not smoke. A short rest is recommended.

Medications and supplements:

Blood samples should be collected before the morning dose. Some drugs can interfere with test results. Consult with your doctor whether you can delay your dose because of lab tests.

Biotin supplements:

High doses of biotin supplements can affect test results, causing false elevation or reduction. It's recommended to avoid taking biotin for at least 72 hours before blood collection. If you are taking biotin, inform the personnel collecting the blood so that they can provide specific instructions.

Interpreting Test Results

Understanding test results for amoebic antibodies is crucial for diagnosing infections caused by *Entamoeba histolytica*. This test is particularly useful for identifying extraintestinal amebiasis. Here's a brief guide:

- Negative: No antibodies detected; no current or past infection.
- Equivocal: Inconclusive; consider retesting in 10-14 days.
- Positive: Indicates current or past infection; further testing recommended for acute diagnosis.

Remember, serology aids diagnosis but should be interpreted alongside clinical findings and other tests for accurate results.

Price of test:£65

The lowest price from the last 30 days: £65

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