The glutathione test is a diagnostic tool used to assess the redox status of red blood cells (RBCs) by measuring glutathione levels in whole blood. Glutathione, a tripeptide composed of cysteine, glycine, and glutamic acid, plays a vital role in protecting cells from oxidative stress. The test requires a whole blood specimen collected in a yellow-top ACD-B or ACD-A tube, with a minimum volume of 100 μL. Proper specimen handling is crucial, as hemolyzed or clotted samples are rejected. The specimen remains stable at room temperature or refrigerated for up to 14 days, but only 7 days if frozen. Utilizing liquid chromatography/tandem mass spectrometry (LC/MS-MS), the test provides precise quantification of both reduced and oxidized glutathione forms. Clinically, it aids in diagnosing conditions like neonatal hyperbilirubinemia, favism, and glutathione synthetase deficiency. While offering valuable insights, results should be interpreted cautiously and confirmed with additional diagnostic procedures.
The glutathione test is a diagnostic tool used to assess the redox status of red blood cells (RBCs) by measuring glutathione levels in whole blood. Glutathione, a tripeptide composed of cysteine, glycine, and glutamic acid, plays a vital role in protecting cells from oxidative stress. The test requires a whole blood specimen collected in a yellow-top ACD-B or ACD-A tube, with a minimum volume of 100 μL. Proper specimen handling is crucial, as hemolyzed or clotted samples are rejected. The specimen remains stable at room temperature or refrigerated for up to 14 days, but only 7 days if frozen. Utilizing liquid chromatography/tandem mass spectrometry (LC/MS-MS), the test provides precise quantification of both reduced and oxidized glutathione forms. Clinically, it aids in diagnosing conditions like neonatal hyperbilirubinemia, favism, and glutathione synthetase deficiency. While offering valuable insights, results should be interpreted cautiously and confirmed with additional diagnostic procedures.
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Glutathione, a tripeptide of cysteine, glycine, and glutamic acid, is vital for maintaining cellular redox balance. In red blood cells, it primarily exists in its reduced form (GSH), protecting against oxidative stress. Measuring glutathione in whole blood assesses RBC redox status, offering insights into health conditions.
- Specimen Requirements: Collect in a yellow-top ACD tube; invert 8-10 times. Minimum 100 μL, preferred 500 μL.
- Storage: Room temperature or refrigerated for 14 days; frozen for 7 days.
- Clinical Significance: Diagnoses neonatal hyperbilirubinemia, favism, hemolysis, and glutathione synthetase deficiency.
A blood glucose test measures the glucose level in the blood, crucial for diagnosing and managing diabetes. It helps healthcare providers monitor disease progression and treatment effectiveness. Key types of tests include:
- Fasting Blood Glucose Test: Requires 8-hour fasting; normal levels are 70-100 mg/dL.
- Random Blood Glucose Test: No fasting needed; levels of 200 mg/dL or higher may indicate diabetes.
- Oral Glucose Tolerance Test (OGTT): Involves drinking a sugary liquid; useful for diagnosing gestational diabetes.
- Hemoglobin A1c Test: Assesses average blood sugar over 2-3 months; levels of 6.5% or higher indicate diabetes.
Monitoring blood glucose is vital for preventing complications.
Understanding test results for glutathione levels in whole blood is crucial for evaluating cellular redox status and potential oxidative stress. Key points include:
- Normal Values: No established reference for patients under 12 months. Healthy individuals typically have reduced glutathione (GSH) levels less than 25% of the mean normal value in certain deficiencies.
- Elevated Levels: May indicate conditions like pyrimidine-5’-nucleotidase deficiency or lead poisoning but can be nonspecific.
- Interpretation Caution: High white blood cell counts can falsely elevate results by up to 25%.
- Limitations: Results are investigational and should be confirmed with other diagnostic methods.
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