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Horseradish IgE

Horseradish IgE

Horseradish peroxidase (HRP)-conjugated antibodies, especially those targeting IgG, are pivotal in laboratory techniques like immunohistochemistry, Western blotting, and ELISAs. These antibodies facilitate the detection and visualization of specific proteins by binding to target antigens and catalyzing a reaction with substrates like 3,3'-diaminobenzidine (DAB), resulting in a visible precipitate. The intensity of this precipitate correlates with the amount of antibody bound, enabling precise quantification of protein expression. In immunohistochemistry, HRP-conjugated antibodies help visualize proteins in tissue sections, aiding disease diagnosis. In Western blotting, they confirm protein presence and size by producing colored bands on membranes. ELISAs utilize these antibodies to quantify proteins or antibodies in samples, with color intensity indicating antigen levels. HRP-conjugated antibodies are species-specific, minimizing cross-reactivity, and are prepared using a modified Nakane and Kawaoi procedure. Their versatility and specificity make them essential tools in molecular biology and immunology research.

Price of test:£34

The lowest price from the last 30 days: £34

Awaiting result:

One day

Collect material:

Blood

Prepare:

Learn more

Purpose of the test

Horseradish peroxidase (HRP)-conjugated antibodies are pivotal in laboratory settings for detecting and visualizing proteins. These antibodies, targeting IgG, are integral to techniques like immunohistochemistry, Western blotting, and ELISAs. The detection mechanism involves HRP catalyzing substrate oxidation, producing a colored product proportional to protein presence.

Applications include:

- Immunohistochemistry (IHC): Visualizes proteins in tissue sections.
- Western Blotting: Confirms protein presence and size.
- ELISA: Quantifies proteins or antibodies in samples.

HRP-conjugated antibodies offer specificity, minimizing cross-reactivity, and are prepared for stable use. Optimal dilution varies by application.

Who Should Get Tested

The Horseradish IgE test is essential for diagnosing horseradish allergies. It measures IgE antibodies, indicating allergic reactions. Key candidates for testing include:

- Individuals with suspected horseradish allergies
- Those experiencing symptoms like skin rashes, itching, or respiratory issues after consuming horseradish

The test involves:

- Blood collection and serum preparation
- Enzyme immunoassay to detect IgE levels

Results are categorized into classes, aiding diagnosis. While valuable, results should be considered alongside clinical symptoms for accurate diagnosis. This test complements other methods, offering reliable insights for allergy management.

Blood Test Preparation Guidelines

Time of day

It is recommended to schedule your appointment for blood tests in the morning hours between 7:00-10:00.

Fasting

It is recommended to fast for approximately 12 hours before blood sampling. The last meal of the previous day should ideally be consumed around 6:00 p.m. On the day before the test, avoid heavy and fatty meals as well as alcohol.

Stay hydrated

Drinking water prior to testing can help with sample collection.

Other factors

Tests should not be performed after a sleepless night or intense physical activity. It is recommended to avoid exercise and stress immediately before blood collection and to not smoke. A short rest is recommended.

Medications and supplements:

Blood samples should be collected before the morning dose. Some drugs can interfere with test results. Consult with your doctor whether you can delay your dose because of lab tests.

Biotin supplements:

High doses of biotin supplements can affect test results, causing false elevation or reduction. It's recommended to avoid taking biotin for at least 72 hours before blood collection. If you are taking biotin, inform the personnel collecting the blood so that they can provide specific instructions.

Interpreting Test Results

Understanding test results involving HRP-conjugated antibodies is essential for accurate data interpretation. These antibodies, targeting IgG, are pivotal in detecting proteins across various applications. Key points include:

- Detection Mechanism: HRP catalyzes substrate oxidation, forming a colored product. The intensity correlates with antibody-antigen binding, enabling protein quantification.

- Applications:
- Immunohistochemistry: Visualizes proteins in tissues, aiding disease diagnosis.
- Western Blotting: Confirms protein presence and size.
- ELISA: Quantifies proteins/antibodies in samples.

- Specificity: Designed to minimize cross-reactivity, ensuring precise detection.

Understanding these aspects ensures reliable and meaningful test outcomes.

Price of test:£34

The lowest price from the last 30 days: £34

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